TIMP2 anticorps (N-Term)

N° du produit ABIN6940735

Cet anticorps anti-TIMP2 est un anticorps Souris Monoclonal détectant TIMP2 dans WB, IHC, IF, FACS et StM. Adapté pour Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin et Cobaye.

Aperçu rapide pour TIMP2 anticorps (N-Term) (ABIN6940735)

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIMP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 3A4

Détail du produit anti-TIMP2 anticorps

Épitope: AA 56-66, N-Term

Specificité: It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Purification: Purified by Protein A/G

Immunogène: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TIMP2

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Autre désignation: TIMP2

Poids moléculaire: 21kDa

ID gène: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process