Recombinant TYMS anticorps (AA 60-174)

N° du produit ABIN6940823

Cet anticorps anti-TYMS est un anticorps Souris Monoclonal détectant TYMS dans IF, FACS, ELISA, RIA et Coat. Adapté pour Humain.

Aperçu rapide pour Recombinant TYMS anticorps (AA 60-174) (ABIN6940823)

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYMS est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Radioimmunoassay (RIA), Coating (Coat)

Clone: RTYMS-1884

Détail du produit anti-TYMS anticorps

Épitope: AA 60-174

Specificité: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Purification: Purified by Protein A/G

Immunogène: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TYMS

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Autre désignation: TYMS

Poids moléculaire: 36kDa

ID gène: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases