Vinculin anticorps (AA 174-322)

N° du produit ABIN6940866

Cet anticorps Souris Monoclonal détecte spécifiquement Vinculin dans WB, IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Vinculin anticorps (AA 174-322) (ABIN6940866)

Antigène: Vinculin (VCL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vinculin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: VCL-2575

Détail du produit anti-Vinculin anticorps

Épitope: AA 174-322

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: A431, K562, NIH3T3, U-87, THP-1 cells. Human Bladder and Testis.

Known Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Vinculin

Antigène: Vinculin (VCL)

Autre désignation: VCL

Sujet: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin. In addition, vinculin, Talin and -Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Poids moléculaire: 117kDa

ID gène: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2