Recombinant Villin 1 anticorps (AA 179-311)

N° du produit ABIN6940874

Cet anticorps Souris Monoclonal détecte spécifiquement Villin 1 dans IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Villin 1 anticorps (AA 179-311) (ABIN6940874)

Antigène: Villin 1 (VIL1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Villin 1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RVIL1-1325

Détail du produit anti-Villin 1 anticorps

Épitope: AA 179-311

Specificité: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Purification: Purified by Protein A/G

Immunogène: A recombinant fragment (around aa179-311) of human Villin protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Villin 1

Antigène: Villin 1 (VIL1)

Autre désignation: VIL1

Poids moléculaire: 93kDa

ID gène: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization