Vimentin anticorps

N° du produit ABIN6940879

Cet anticorps Souris Monoclonal détecte spécifiquement Vimentin dans WB, IHC, FACS et StM. Il présente une réactivité avec des échantillons de Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat et Chévre.

Aperçu rapide pour Vimentin anticorps (ABIN6940879)

Antigène: Vimentin (VIM)

Reactivité: Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: VM452

Détail du produit anti-Vimentin anticorps

Specificité: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human vimentin protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: VIM

Poids moléculaire: 57-60kDa

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis