Recombinant WT1 anticorps

N° du produit ABIN6940905

Cet anticorps anti-WT1 est un anticorps Lapin Monoclonal détectant WT1 dans IHC et StM. Adapté pour Humain.

Aperçu rapide pour Recombinant WT1 anticorps (ABIN6940905)

Antigène: WT1 (Wilms Tumor 1 (WT1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp WT1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: WT1-1434R

Détail du produit anti-WT1 anticorps

Specificité: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length WT1 protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: K562 cells. Wilm's Tumor, mesothelioma or fetal kidney.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur WT1

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: WT1

Poids moléculaire: 47-55kDa

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation