Caldesmon anticorps

N° du produit ABIN6940938

L’anticorps anti-Caldesmon Monoclonal Souris est utilisé pour la détection de Caldesmon dans des échantillons de Humain. Il a été validé pour WB, IHC, IF et FACS.

Aperçu rapide pour Caldesmon anticorps (ABIN6940938)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: H-CALD

Détail du produit anti-Caldesmon anticorps

Fonction: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

Specificité: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Crude human uterus extract

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: CALD1

Sujet:

CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Blood vessels in all tissues, smooth muscle or leiomyosarcoma., Uterus

Poids moléculaire: 150kDa

ID gène: 800, 490203

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction