TIM3 anticorps (AA 22-202)

N° du produit ABIN6940963

L’anticorps Souris Monoclonal anti-TIM3 a été validé pour ELISA, IHC, Coat et StM. Il convient pour détecter TIM3 dans des échantillons de Humain.

Aperçu rapide pour TIM3 anticorps (AA 22-202) (ABIN6940963)

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIM3 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Clone: TIM3-3113

Détail du produit anti-TIM3 anticorps

Épitope: AA 22-202

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human TIM3 protein (around aa 22-202) (exact sequence is proprietary)

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: PC3, BT474, HepG2, HDLM-2 or Daudi cells. Tonsil, lymph node or spleen.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TIM3

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Autre désignation: HAVCR2

Classe de substances: Virus

Sujet: TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.

Poids moléculaire: 60kDa

ID gène: 84868

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints