p63 anticorps (AA 5-17)

N° du produit ABIN6940969

Cet anticorps Lapin Polyclonal détecte spécifiquement p63 dans WB, IHC et StM. Il présente une réactivité envers Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour p63 anticorps (AA 5-17) (ABIN6940969)

Antigène: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p63 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Détail du produit anti-p63 anticorps

Épitope: AA 5-17

Immunogène: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur p63

Antigène: p63 (TP63) (Tumor Protein P63 (TP63))

Autre désignation: TP63

Sujet: P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Poids moléculaire: 40kDa

ID gène: 8626

UniProt: Q9H3D4