PDLIM1 anticorps

N° du produit ABIN6941009

Cet anticorps anti-PDLIM1 est un anticorps Souris Monoclonal détectant PDLIM1 dans WB, IHC et StM. Adapté pour Humain.

Aperçu rapide pour PDLIM1 anticorps (ABIN6941009)

Antigène: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDLIM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CPTC-PDLIM1-1

Détail du produit anti-PDLIM1 anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length PDLIM1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur PDLIM1

Antigène: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Autre désignation: PDLIM1

Sujet: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species.

Poids moléculaire: 36kDa

ID gène: 9124

UniProt: O00151