CD5 anticorps (AA 450-495)

N° du produit ABIN6941052

Cet anticorps Souris Monoclonal détecte spécifiquement CD5 dans FACS, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Rat, Boeuf (Vache), Lapin, Cheval, Porc, Singe, Poulet et Cobaye.

Aperçu rapide pour CD5 anticorps (AA 450-495) (ABIN6941052)

Antigène: CD5

Reactivité: Humain, Rat, Boeuf (Vache), Lapin, Cheval, Porc, Singe, Poulet, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: CD5-54-F6

Détail du produit anti-CD5 anticorps

Épitope: AA 450-495

Fonction: CD5 (Mantle Cell Lymphoma Marker) Antibody

Specificité: Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region).

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet:

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

293T, Ramos or MOLT-4 cells. Human tonsil.

Poids moléculaire: ~67kDa

ID gène: 921, 58685

UniProt: P06127