CD22 anticorps (AA 52-178)

N° du produit ABIN6941121

Détail du produit anti-CD22 anticorps

Antigène: CD22 (CD22 Molecule (CD22))

Épitope: AA 52-178

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD22 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat)

Specificité: Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human CD22 protein (around aa 52-178) (exact sequence is proprietary)

Clone: BLCAM-1796

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: Raji or Ramos cells (IF/FACS). Raji cell lysate (WB). Human tonsil, lymph node or spleen (IHC).

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD22

Antigène: CD22 (CD22 Molecule (CD22))

Autre désignation: CD22 (CD22 Produits)

Synonymes: anticorps A530093D23, anticorps Lyb-8, anticorps Lyb8, anticorps SIGLEC-2, anticorps SIGLEC2, anticorps siglec-2, anticorps CD22 antigen, anticorps CD22 molecule, anticorps Cd22, anticorps CD22

Poids moléculaire: 130-140kDa

ID gène: 933

UniProt: P20273