Phosphotyrosine anticorps

N° du produit ABIN6941322

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphotyrosine dans WB, IP, IF, FACS, IHC, ISt et StM. Il présente une réactivité envers .

Aperçu rapide pour Phosphotyrosine anticorps (ABIN6941322)

Antigène: Phosphotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: PY20

Détail du produit anti-Phosphotyrosine anticorps

Purification: Purified by Protein A/G

Immunogène: Phosphotyrosine conjugated to KLH

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: MCF-7, MDA-231, T47-D cells. Breast carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Autre désignation: Phosphotyrosine (P-Tyr)

Classe de substances: Amino Acid

Sujet: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.