CGA (HCG-Alpha) & CGB (HCG-Beta) anticorps

N° du produit ABIN6941346

Cet anticorps anti-CGA (HCG-Alpha) & CGB (HCG-Beta) Monoclonal Souris (Clone HCGab-52) (ABIN6941346) détecte spécifiquement CGA (HCG-Alpha) & CGB (HCG-Beta) dans IHC et StM. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CGA (HCG-Alpha) & CGB (HCG-Beta) anticorps (ABIN6941346)

Antigène: CGA (HCG-Alpha) & CGB (HCG-Beta)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: HCGab-52

Détail du produit

Purification: Purified by Protein A/G

Immunogène: Purified hCG protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: JAR or TT cells. Placenta.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: CGA (HCG-Alpha) & CGB (HCG-Beta)

Sujet: This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Poids moléculaire: ~35kDa (intact hCG)

ID gène: 1081, 1082

UniProt: P01215, P01233