KRT anticorps

N° du produit ABIN6941353

Cet anticorps Souris Monoclonal détecte spécifiquement KRT dans FACS, IHC et StM. Il présente une réactivité envers Humain, Souris, Rat, Chien, Poulet, Boeuf (Vache), Singe et Lapin.

Aperçu rapide pour KRT anticorps (ABIN6941353)

Antigène: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Reactivité: Humain, Souris, Rat, Chien, Poulet, Boeuf (Vache), Singe, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: Cocktail PAN-CK

Détail du produit anti-KRT anticorps

Specificité: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Purification: Purified by Protein A/G

Immunogène: Human epidermal keratins

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur KRT

Antigène: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Autre désignation: KRT

Poids moléculaire: 40-67kDa (Multiple)

ID gène: 374454, 51350

UniProt: Q7Z794, Q01546