Melanoma Associated Antigen (KBA.62) anticorps

N° du produit ABIN6941354

Cet anticorps anti-Melanoma Associated Antigen (KBA.62) Monoclonal Souris (Clone KBA-62) (ABIN6941354) détecte spécifiquement Melanoma Associated Antigen (KBA.62) dans IF et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Melanoma Associated Antigen (KBA.62) anticorps (ABIN6941354)

Antigène: Melanoma Associated Antigen (KBA.62)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: KBA-62

Détail du produit

Fonction: KBA.62 (Melanoma Associated Antigen) Antibody

Specificité: KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Human KAL cells derived from lymph node metastasis of malignant melanoma

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Melanoma Associated Antigen (KBA.62)

Autre désignation: KBA.62 (Melanoma Associated Antigen)

Sujet:

Human Melanoma Associated Antigen, KBA.62

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Poids moléculaire: 135 and 128kDa and two weak bands of 88 and 73kDa)| (140