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Recombinant Cytokeratin, Multi (Epithelial Marker) anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement dans IHC et StM. Il présente une réactivité envers Boeuf (Vache), Grenouille, Chévre, Cobaye, Humain, Singe, Souris, Porc et Rat.
N° du produit ABIN6941405

Aperçu rapide pour Recombinant Cytokeratin, Multi (Epithelial Marker) anticorps (ABIN6941405)

Antigène

Cytokeratin, Multi (Epithelial Marker)

Type d'anticorp

Recombinant Antibody

Reactivité

Boeuf (Vache), Grenouille, Chévre, Cobaye, Humain, Singe, Souris, Porc, Rat

Hôte

  • 2
  • 1
Souris

Clonalité

  • 3
Monoclonal

Conjugué

  • 3
Inconjugué

Application

  • 3
  • 3
  • 2
  • 2
  • 2
Immunohistochemistry (IHC), Staining Methods (StM)

Clone

RKRT-457
  • Specificité

    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

    Purification

    Purified by Protein A/G

    Immunogène

    Keratin-enriched preparation from cultured human epithelial cells

    Isotype

    IgG1 kappa
  • Indications d'application

    Positive Control: A431 cells, Skin, Colon carcinoma.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Date de péremption

    24 months
  • Antigène

    Cytokeratin, Multi (Epithelial Marker)

    ID gène

    3851, 3852, 3853

    UniProt

    P02538, P04259, P13647, P19013, P48668
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