KRT anticorps

N° du produit ABIN6941421

L’anticorps Souris Monoclonal anti-KRT a été validé pour FACS, IHC, IF et StM. Il convient pour détecter KRT dans des échantillons de Humain, Chien et Cat.

Aperçu rapide pour KRT anticorps (ABIN6941421)

Antigène: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Reactivité: Humain, Chien, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: PCK-3150

Détail du produit anti-KRT anticorps

Purification: Purified by Protein A/G

Immunogène: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: Skin, adeno- or squamous carcinomas (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur KRT

Antigène: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Autre désignation: KRT

Sujet: Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Poids moléculaire: 40-67kDa (Multiple)