Recombinant Keratin 77 anticorps
Aperçu rapide pour Recombinant Keratin 77 anticorps (ABIN6941453)
Antigène
Voir toutes Keratin 77 (KRT77) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody
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Specificité
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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Réactivité croisée (Details)
- Expected to show broad species reactivity.
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Purification
- 200ug/ml of Ab Purified by Protein A/G.
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Immunogène
- Recombinant human KRT77 protein fragment
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Isotype
- IgG, kappa
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Indications d'application
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Positive Control: Skin, Squamous cell carcinoma (SCC).
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Date de péremption
- 24 months
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- Keratin 77 (KRT77)
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Autre désignation
- KRT77
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Sujet
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K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B
Skin, Squamous cell carcinoma (SCC). A431.
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Poids moléculaire
- 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
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ID gène
- 374454, 334989
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UniProt
- Q7Z794
Antigène
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