FTO anticorps

N° du produit ABIN6943480

L’anticorps Lapin Monoclonal anti-FTO a été validé pour WB, IHC (p), IF (cc), IF (p) et FACS. Il convient pour détecter FTO dans des échantillons de Humain.

Aperçu rapide pour FTO anticorps (ABIN6943480)

Antigène: FTO (Fat Mass and Obesity-Associated (FTO))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp FTO est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Clone: 12A1

Détail du produit anti-FTO anticorps

Réactivité croisée: Humain

Purification: Purified by Protein A.

Immunogène: Recombinant protein within human FTO aa 350-500

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 12 months

Détails sur FTO

Antigène: FTO (Fat Mass and Obesity-Associated (FTO))

Autre désignation: FTO

Sujet:

Synonyms: Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, FTO, KIAA1752, GDFD, ALKBH9, BMIQ14, Fatso.

Background: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Also acts as a tRNA demethylase by removing N1-methyladenine from various tRNAs. Also able to repair alkylated DNA and RNA by oxidative demethylation. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression

ID gène: 79068

UniProt: Q9C0B1