MAD2L1 anticorps

N° du produit ABIN6943982

Cet anticorps anti-MAD2L1 est un anticorps Lapin Monoclonal détectant MAD2L1 dans WB, IF (cc), IF (p) et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour MAD2L1 anticorps (ABIN6943982)

Antigène: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MAD2L1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6C6

Détail du produit anti-MAD2L1 anticorps

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: Recombinant protein within human Mad2L1 aa 1-150

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur MAD2L1

Antigène: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Autre désignation: Mad2L1

Sujet:

Synonyms: Mitotic spindle assembly checkpoint protein MAD2A, Mitotic arrest deficient 2-like protein 1, HsMAD2, MAD2-like protein 1, MAD2L1, MAD2.

Background: Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase.

ID gène: 4085

UniProt: Q13257