PUM1 anticorps (AA 1-200)

N° du produit ABIN6944728

L’anticorps anti-PUM1 Monoclonal Lapin est utilisé pour la détection de PUM1 dans des échantillons de Humain. Il a été validé pour WB, FACS, IHC (p), IF (cc) et IF (p).

Aperçu rapide pour PUM1 anticorps (AA 1-200) (ABIN6944728)

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PUM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 7B2

Détail du produit anti-PUM1 anticorps

Épitope: AA 1-200

Fonction: Pumilio 1 (7B2) Monoclonal Antibody

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: Recombinant protein within human Pumilio 1 aa 1-200.

Isotype: IgG

Information d'application

Indications d'application: WB(1:300-5000), FCM(1:200-400), IHC-P(1:50-200), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PUM1

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Autre désignation: PUM1

Sujet:

Synonyms: Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, PUMH, HSPUM, PUMH1, PUML1, SCA47

Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. Plays a role in cytoplasmic sensing of viral infection. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.

ID gène: 9698

UniProt: Q14671