PUM1 anticorps

N° du produit ABIN6944728

L’anticorps Lapin Monoclonal anti-PUM1 a été validé pour WB, IHC (p), FACS, IF (cc) et IF (p). Il convient pour détecter PUM1 dans des échantillons de Humain et Souris.

Aperçu rapide pour PUM1 anticorps (ABIN6944728)

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PUM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 7B2

Détail du produit anti-PUM1 anticorps

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: Recombinant protein within human Pumilio 1 aa 1-200.

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PUM1

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Autre désignation: PUM1

Sujet:

Synonyms: Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, PUMH, HSPUM, PUMH1, PUML1, SCA47

Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. Plays a role in cytoplasmic sensing of viral infection. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.

ID gène: 9698

UniProt: Q14671