Recombinant SARS-CoV-2 Spike anticorps (Receptor Binding Domain) (Fc Tag)

N° du produit ABIN6953152

Cet anticorps anti-SARS-CoV-2 Spike est un anticorps Human Monoclonal détectant SARS-CoV-2 Spike dans ELISA, Neut et GICA. Adapté pour SARS Coronavirus-2 (SARS-CoV-2).

Aperçu rapide pour Recombinant SARS-CoV-2 Spike anticorps (Receptor Binding Domain) (Fc Tag) (ABIN6953152)

Antigène: SARS-CoV-2 Spike

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: SARS Coronavirus-2 (SARS-CoV-2)

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp SARS-CoV-2 Spike est conjugé à/à la Fc Tag

Application: ELISA, Neutralization (Neut), Colloidal Gold Immunochromatography Assay (GICA)

Clone: A1

Détail du produit anti-SARS-CoV-2 Spike anticorps (Fc Tag)

Épitope: AA 319-541, Receptor Binding Domain

Fonction: SARS-CoV-2 Spike RBD single-domain Antidbody

Attributs du produit: This SARS-CoV-2 Spike RBD Nanobody is a recombinant monoclonal antibody generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike receptor-binding domain (RBD). The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2-S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA). In neutralization assay, the binding signal of SARS-CoV-2 Spike RBD Nanobody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/mL IC50. Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein (S protein), so the SARS-CoV-2 Spike RBD Nanobody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.
VHH fusion with human IgG1 Fc

Purification: Affinity-chromatography

Immunogène: Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)

Isotype: IgG1

Information d'application

Indications d'application: ELISA:1:10000-1:100000, GICA:1:10000-1:40000, Neutralising:1:100-1:10000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze

Détails sur SARS-CoV-2 Spike

Antigène: SARS-CoV-2 Spike

Autre désignation: SARS-CoV-2 (SARS-CoV-2/ 2019-nCoV) SARS-CoV-2 Spike

Sujet: Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.

ID gène: 43740568

UniProt: P0DTC2