Recombinant SARS-CoV-2 (SARS-CoV-2/ 2019-nCoV) S (AA 16-685) anticorps (Fc Tag,HRP)

N° du produit ABIN6953153

Cet anticorps Souris Monoclonal détecte spécifiquement dans ELISA. Il présente une réactivité envers SARS Coronavirus-2 (SARS-CoV-2).

Aperçu rapide pour Recombinant SARS-CoV-2 (SARS-CoV-2/ 2019-nCoV) S (AA 16-685) anticorps (Fc Tag,HRP) (ABIN6953153)

Antigène: SARS-CoV-2 (SARS-CoV-2/ 2019-nCoV) S

Type d'anticorp: Recombinant Antibody

Fragment: scFv fragment

Reactivité: SARS Coronavirus-2 (SARS-CoV-2)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Fc Tag,HRP

Application: ELISA

Clone: H6

Détail du produit

Épitope: AA 16-685

Fonction: S Recombinant Monoclonal Antibody, HRP conjugated

Attributs du produit: Recombinant anti-SARS-CoV-2 spike Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
Mouse scFv fusion with human IgG1 Fc
AA 16-685

Purification: Affinity-chromatography

Immunogène: Recombinant Human Novel Coronavirus Spike glycoprotein (S) (16-685aa) (CSB-MP3324GMY)

Isotype: IgG1

Information d'application

Indications d'application: ELISA:1:10000-1:50000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze

Détail du antigène

Antigène: SARS-CoV-2 (SARS-CoV-2/ 2019-nCoV) S

Sujet: Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.

ID gène: 43740568

UniProt: P0DTC2