Recombinant GFP anticorps (Alexa Fluor 647)

N° du produit ABIN6953230

L’anticorps Alpaca Monoclonal anti-GFP a été validé pour IF, ICC et FACS. Il convient pour détecter GFP dans des échantillons de Aequorea victoria.

Aperçu rapide pour Recombinant GFP anticorps (Alexa Fluor 647) (ABIN6953230)

Antigène: GFP (Green Fluorescent Protein (GFP))

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Aequorea victoria

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est conjugé à/à la Alexa Fluor 647

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1H1

Détail du produit anti-GFP anticorps (Alexa Fluor 647)

Expression System: E.coli

Fonction: Camelid sdAb anti-GFP conjugated with Alexa647, Clones 1H1

Specificité: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Réactivité croisée (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Attributs du produit: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Information d'application

Indications d'application: Recommended dilution 1:500

Commentaires:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Conseil sur la manipulation: Protect from light!

Stock: -20 °C,-80 °C

Stockage commentaire: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP