Recombinant GFP anticorps (Alexa Fluor 647)

N° du produit ABIN6953232

Détail du produit anti-GFP anticorps (Alexa Fluor 647)

Antigène: GFP (Green Fluorescent Protein (GFP))

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Aequorea victoria

Hôte: Alpaca

Expression System: E.coli

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est conjugé à/à la Alexa Fluor 647

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Fonction: Camelid sdAb anti-GFP conjugated with Alexa647, Clones 1H1/1B2

Specificité: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Réactivité croisée (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Attributs du produit: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Clone: 1H1-1B2

Labeling Ratio: A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.

Information d'application

Indications d'application: Recommended dilution 1:500

Commentaires:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable.

Concentration: 5 μM

Buffer: Lyophilized from PBS pH 7.4.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light!

Stock: -20 °C,-80 °C

Stockage commentaire: Vials containing lyophilized protein can be stored at 4 °C for 6 months. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Date de péremption: 6 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP (GFP Produits)

Synonymes: anticorps green fluorescent protein, anticorps gfp

Classe de substances: Viral Protein