Recombinant Blue Fluorescent Protein anticorps (AZDye 568)

N° du produit ABIN6953241

Détail du produit anti-Blue Fluorescent Protein anticorps (AZDye 568)

Antigène: Blue Fluorescent Protein (BFP)

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Entacmaea quadricolor

Hôte: Alpaca

Expression System: E.coli

Clonalité: Monoclonal

Conjugué: Cet anticorp Blue Fluorescent Protein est conjugé à/à la AZDye 568

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Fonction: Camelid sdAb anti-TagBFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 1H7

Specificité: Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor

Réactivité croisée (Details): Does not cross-react with common GFP- or dsRed derivatives.

Attributs du produit: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Clone: 1H7

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Information d'application

Indications d'application: Recommended dilution 1:500

Commentaires:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA. Please reconstitute with 200 µL of 50 % glycerol in deionized water. It is recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 5 µM.

Concentration: 5 μM

Buffer: 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light!

Stock: -20 °C,-80 °C

Stockage commentaire: Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. It is recommend storing the reconstituted protein at 4 °C.

Date de péremption: 12 months

Détails sur Blue Fluorescent Protein

Antigène: Blue Fluorescent Protein (BFP)

Autre désignation: TagBFP (BFP Produits)