Recombinant mEos anticorps (Atto 488)
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- Antigène
- mEos
- Type d'anticorp
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivité
- Lobophyllia hemprichii
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Hôte
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Alpaca
- Expression System
- E.coli
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Clonalité
- Monoclonal
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Conjugué
- Atto 488
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- Camelid sdAb anti-mEOS conjugated with Atto488, Clone 1E8
- Specificité
- Recognizes mEOS and most common mEOS derivatives
- Attributs du produit
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- Purification
- Produced in: E.coli
- Clone
- 1E8
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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- Indications d'application
- Recommended dilution 1:500
- Commentaires
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light!
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- Date de péremption
- 12 months
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- Antigène
- mEos
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