Recombinant mRuby anticorps (Atto 488)

N° du produit ABIN6953281

L’anticorps Alpaca Monoclonal anti- a été validé pour IF, ICC et FACS. Il convient pour détecter dans des échantillons de Entacmaea quadricolor.

Aperçu rapide pour Recombinant mRuby anticorps (Atto 488) (ABIN6953281)

Antigène: mRuby

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Entacmaea quadricolor

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Atto 488

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1F8

Détail du produit

Expression System: E.coli

Fonction: Camelid sdAb anti-mRuby conjugated with Atto488, Clone 1F8

Specificité: Recognizes mRuby, mRuby3 and common mRuby derivates.

Attributs du produit: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Information d'application

Indications d'application: Recommended dilution 1:500

Commentaires:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Conseil sur la manipulation: Protect from light!

Stock: -20 °C,-80 °C

Stockage commentaire: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Date de péremption: 12 months

Détail du antigène

Antigène: mRuby