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MHC II (I-A/I-E) anticorps (violetFluor™ 450)

L’anticorps Rat Monoclonal anti-MHC II (I-A/I-E) a été validé pour FACS. Il convient pour détecter MHC II (I-A/I-E) dans des échantillons de Souris.
N° du produit ABIN7278570

Aperçu rapide pour MHC II (I-A/I-E) anticorps (violetFluor™ 450) (ABIN7278570)

Antigène

MHC II (I-A/I-E)

Reactivité

  • 37
  • 1
Souris

Hôte

  • 38
Rat

Clonalité

  • 38
Monoclonal

Conjugué

  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp MHC II (I-A/I-E) est conjugé à/à la violetFluor™ 450

Application

  • 36
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

M5-114-15-2
  • Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

    Isotype

    IgG2b kappa
  • Indications d'application

    This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

    Commentaires

    0.2 mg/mL

    Restrictions

    For Research Use only
  • Buffer

    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Stockage commentaire

    2-8°C protected from light

    Date de péremption

    12 months
  • Antigène

    MHC II (I-A/I-E)

    Autre désignation

    MHC Class II (I-A/I-E)

    Sujet

    The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

    ID gène

    14961, 14969

    UniProt

    P14483
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