Interleukin 17a anticorps (FITC)

N° du produit ABIN6963909

L’anticorps Souris Monoclonal anti-Interleukin 17a a été validé pour FACS. Il convient pour détecter Interleukin 17a dans des échantillons de Humain, Boeuf (Vache), Porc, Chien, Mouton, Cheval et Non-Human Primate.

Aperçu rapide pour Interleukin 17a anticorps (FITC) (ABIN6963909)

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivité: Humain, Boeuf (Vache), Porc, Chien, Mouton, Cheval, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Interleukin 17a est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: MT504

Détail du produit anti-Interleukin 17a anticorps (FITC)

Specificité: Native and recombinant IL-17A.

Réactivité croisée (Details): The antibody also cross-reacts with IL-17A from cow, sheep, horse, pig and dog.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant human IL-17A

Isotype: IgG1

Information d'application

Indications d'application: Detection of IL-17A producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-17A by flow cytometry in human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-human IL-17A mAb MT504-FITC and anti-human IFN-γ-PE (right panel). The left panel depicts stimulated cells stained with matched isotype control antibodies. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur Interleukin 17a

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Autre désignation: IL17A

ID gène: 3605, 282863, 100034142