Histone H1 anticorps (AA 109-256)

N° du produit ABIN6971739

Détail du produit anti-Histone H1 anticorps

Antigène: Histone H1

Épitope: AA 109-256

Reactivité: Drosophila melanogaster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H1 est non-conjugé

Application: Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Attributs du produit: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression. Histone H1 antibody (mAb) (Clone 1G6-E3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Drosophila samples.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals, the antibody is not likely to cross-react to human, mouse or rat Histone H1. 

Clone: 1G6-E3

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone H1

Antigène: Histone H1

Abstract: Histone H1 Produits

Synonymes: anticorps H10, anticorps H1FV, anticorps H1 histone family member 0, anticorps H1F0

Poids moléculaire: 35 kDa

NCBI Accession: NP_001027286