Histone H3.1 anticorps (AA 21-39)

N° du produit ABIN6971835

Détail du produit anti-Histone H3.1 anticorps

Antigène: Histone H3.1 (HIST1H3B)

Épitope: AA 21-39

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H3.1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Attributs du produit: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine. Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a peptide comprising amino acids 21-39 of human Histone H3.1. This region is 100% identical in human Histone H3.2.

Clone: 1D4F2

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone H3.1

Antigène: Histone H3.1 (HIST1H3B)

Autre désignation: Histone H3.1 / 3.2 (HIST1H3B Produits)

Synonymes: anticorps H3 histone, anticorps Hist1h2ai, anticorps histone cluster 1, H2ai-like1, anticorps histone H3.1, anticorps Hht21p, anticorps Hist1h2ail1, anticorps H3.1, anticorps HHT21

Poids moléculaire: 17 kDa

NCBI Accession: NP_003522