Histone H3.3 anticorps (pSer31)

N° du produit ABIN6971843

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone H3.3 dans WB, IF et DB. Il présente une réactivité envers Humain.

Aperçu rapide pour Histone H3.3 anticorps (pSer31) (ABIN6971843)

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H3.3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Détail du produit anti-Histone H3.3 anticorps

Épitope: pSer31

Attributs du produit: Histone H3.3 (H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes. (Reference: Hake et al. (2005) PNAS 102: 6344-6349.) Histone H3.3S31ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogène: This Histone H3.3 phospho Ser31 antibody was raised against a peptide containing phospho Ser31 of Histone H3.3.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone H3.3

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Autre désignation: Histone H3.3

Poids moléculaire: 17 kDa

NCBI Accession: NP_002908