Histone H4 anticorps (acLys16)

N° du produit ABIN6972111

L’anticorps Lapin Polyclonal anti-Histone H4 a été validé pour WB, IHC, ChIP et DB. Il convient pour détecter Histone H4 dans des échantillons de Humain, Saccharomyces cerevisiae et Drosophila melanogaster.

Aperçu rapide pour Histone H4 anticorps (acLys16) (ABIN6972111)

Antigène: Histone H4

Reactivité: Humain, Saccharomyces cerevisiae, Drosophila melanogaster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Détail du produit anti-Histone H4 anticorps

Épitope: acLys16

Attributs du produit: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. NoRC is a SMARCA5 (SNF2h)-containing chromatin remodeling complex. The bromodomain of TIP5, the large subunit of NoRC, interacts with acetylated Histone H4 Lys16, (H4K16ac) and cooperates with an adjacent PHD finger to recruit histone deacetylases and DNA methyltransferases to rDNA, leading to the silencing of rDNA. Histone H4K16ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunohistochemistry and Western blot, it has been shown to react with Budding Yeast, Drosophila and Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogène: This Histone H4 acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of histone H4.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone H4

Antigène: Histone H4

Poids moléculaire: 8 kDa

NCBI Accession: NP_778224