Vimentin anticorps (AA 371-466) (AbBy Fluor® 750)

N° du produit ABIN6982480

Cet anticorps Lapin Polyclonal détecte spécifiquement Vimentin dans WB, FACS, IF (p) et IF (cc). Il présente une réactivité envers Humain, Souris, Rat et Lapin.

Aperçu rapide pour Vimentin anticorps (AA 371-466) (AbBy Fluor® 750) (ABIN6982480)

Antigène: Vimentin (VIM)

Reactivité: Humain, Souris, Rat, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Vimentin est conjugé à/à la AbBy Fluor® 750

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Détail du produit anti-Vimentin anticorps (AbBy Fluor® 750)

Épitope: AA 371-466

Specificité: KO-Validated

Réactivité croisée: Humain, Souris, Lapin, Rat

Homologie: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Vimentin

Isotype: IgG

Information d'application

Indications d'application: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet:

Synonyms: VIM, FLJ36605, OTTHUMP00000019224, VIM, VIME_HUMAN, Vimentin.

Background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Pathways: Caspase Cascade in Apoptosis