TAP Tag anticorps (HRP)

N° du produit ABIN6987469

Détail du produit

Antigène: TAP Tag

Reactivité: Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: HRP

Application: Western Blotting (WB), ELISA

Réactivité croisée: Tag

Réactivité croisée (Details): TAP-Tag

Purification: Purified by Protein A.

Immunogène: Recombinant TAP-Tag

Clone: Mix-mA

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: TAP Tag

Autre désignation: TAP-Tag

Classe de substances: Tag

Sujet:

Synonyms: TAP Tag, TAPTag, Tandem affinity purification

Background: Tandem affinity purification (TAP) is a purification technique for studying proteinprotein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.