IKAP/p150 anticorps (C-Term)

N° du produit ABIN6990353

L’anticorps anti-IKAP/p150 Polyclonal Lapin est utilisé pour la détection de IKAP/p150 dans des échantillons de Humain et Souris. Il a été validé pour WB, ELISA, ICC et IF.

Aperçu rapide pour IKAP/p150 anticorps (C-Term) (ABIN6990353)

Antigène: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IKAP/p150 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-IKAP/p150 anticorps

Épitope: C-Term

Specificité: At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.

Purification: IKAP Antibody is affinity chromatography purified via peptide column.

Immunogène: IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP.

Isotype: IgG

Information d'application

Indications d'application: IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: IKAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: IKAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur IKAP/p150

Antigène: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Autre désignation: IKAP

Sujet: IKAP Antibody: IKAP was initially identified as a scaffold protein of the Iκ,B kinase complex that could bind to IKKα, IKKβ, NF-κ,B, and the NF-κ,B-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

Poids moléculaire: Predicted: 134, 147 kDa

Observed: 105, 145 kDa

ID gène: 8518

UniProt: O95163