Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Purification
Nudel Antibody is affinity chromatography purified via peptide column.
Immunogène
Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel. The immunogen is located within the first 50 amino acids of Nudel.
Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Nudel Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
Nudel antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
NDEL1
(NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1 (NDEL1))
anticorps EOPA, anticorps MITAP1, anticorps NDE1L1, anticorps NDE2, anticorps NUDEL, anticorps NUDE2, anticorps Nudel, anticorps 2600006O07Rik, anticorps mNudel, anticorps wu:fi23c04, anticorps wu:fi23e06, anticorps eopa, anticorps mitap1, anticorps ndel1, anticorps ndel1-a, anticorps ndel1-b, anticorps nudel, anticorps nudE neurodevelopment protein 1 like 1, anticorps nudE neurodevelopment protein 1-like 1, anticorps nudE neurodevelopment protein 1-like 1b, anticorps nudE neurodevelopment protein 1 like 1 L homeolog, anticorps NDEL1, anticorps Ndel1, anticorps ndel1b, anticorps ndel1.L
Sujet
Nudel Antibody: Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration, phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.