ST8SIA4 anticorps (N-Term)

N° du produit ABIN6990643

Cet anticorps Lapin Polyclonal détecte spécifiquement ST8SIA4 dans WB et ELISA. Il présente une réactivité envers Saccharomyces cerevisiae.

Aperçu rapide pour ST8SIA4 anticorps (N-Term) (ABIN6990643)

Antigène: ST8SIA4 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ST8SIA4 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-ST8SIA4 anticorps

Épitope: AA 80-130, N-Term

Purification: Pst1 Antibody is affinity chromatography purified via peptide column.

Immunogène: Pst1 antibody was raised against a synthetic peptide from the amino terminus of yeast Pst1. The immunogen is located within amino acids 80 - 130 of Pst1.

Isotype: IgG

Information d'application

Indications d'application: Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 μ,g/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Pst1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: Pst1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur ST8SIA4

Antigène: ST8SIA4 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4))

Autre désignation: Pst1

Sujet: Pst1 Antibody: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δ,och1 Δ,mnn1 Δ,mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.

Poids moléculaire: 49 kDa

ID gène: 851625

UniProt: Q12355