At least two isoforms of Bfl-1 are known to exist, this antibody will detect both isoforms.
Purification
Bfl-1 Antibody is affinity chromatography purified via peptide column.
Immunogène
Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Bfl-1. The immunogen is located within the first 50 amino acids of Bfl-1.
Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Bfl-1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
Bfl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
anticorps ACC-1, anticorps ACC-2, anticorps BCL2L5, anticorps BFL1, anticorps GRS, anticorps HBPA1, anticorps Bcl2a1d, anticorps BCL2A1, anticorps BCL2 related protein A1, anticorps BCL2-related protein A1, anticorps BCL2A1, anticorps Bcl2a1
Sujet
Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.