BRCC3 anticorps (N-Term)

N° du produit ABIN6990795

Cet anticorps anti-BRCC3 est un anticorps Lapin Polyclonal détectant BRCC3 dans WB, ELISA, IHC (p) et IF. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour BRCC3 anticorps (N-Term) (ABIN6990795)

Antigène: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRCC3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-BRCC3 anticorps

Épitope: AA 20-70, N-Term

Purification: BRCC36 Antibody is affinity chromatography purified via peptide column.

Immunogène: BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36.

Isotype: IgG

Information d'application

Indications d'application: BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: BRCC36 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: BRCC36 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur BRCC3

Antigène: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Autre désignation: BRCC36

Sujet: BRCC36 Antibody: Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Poids moléculaire: Predicted: 35 kDa

Observed: 35 kDa

ID gène: 79184

NCBI Accession: NP_077308

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus