ASAH2 anticorps (C-Term)

N° du produit ABIN6990954

Cet anticorps anti-ASAH2 est un anticorps Lapin Polyclonal détectant ASAH2 dans WB et ELISA. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour ASAH2 anticorps (C-Term) (ABIN6990954)

Antigène: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASAH2 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-ASAH2 anticorps

Épitope: C-Term

Purification: ASAH2 Antibody is affinity chromatography purified via peptide column.

Immunogène: ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2. The immunogen is located within the last 50 amino acids of ASAH2.

Isotype: IgG

Information d'application

Indications d'application: ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: ASAH2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: ASAH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur ASAH2

Antigène: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Autre désignation: ASAH2

Sujet: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

ID gène: 56624

NCBI Accession: NP_063946

UniProt: Q9NR71