PIGY anticorps (C-Term)

N° du produit ABIN6991028

L’anticorps Lapin Polyclonal anti-PIGY a été validé pour ELISA, WB, IHC (p) et IF. Il convient pour détecter PIGY dans des échantillons de Humain et Souris.

Aperçu rapide pour PIGY anticorps (C-Term) (ABIN6991028)

Antigène: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIGY est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-PIGY anticorps

Épitope: C-Term

Specificité: This antibody only detects isoform 1 of PIG-Y.

Purification: PIG-Y Antibody is affinity chromatography purified via peptide column.

Immunogène: PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y. The immunogen is located within the last 50 amino acids of PIG-Y.

Isotype: IgG

Information d'application

Indications d'application: PIG-Y antibody can be used for detection of PIG-Y by Western blot at 5 μ,g/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PIG-Y Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur PIGY

Antigène: PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))

Autre désignation: PIG-Y

Sujet: PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

Poids moléculaire: Predicted: 8 kDa

Observed: 9 kDa

ID gène: 84992

NCBI Accession: NP_116295

UniProt: Q3MUY2

Pathways: Inositol Metabolic Process