PIGY anticorps (AA 50-100)
Aperçu rapide pour PIGY anticorps (AA 50-100) (ABIN6991033)
Antigène
Voir toutes PIGY AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 50-100
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Specificité
- This antibody only detects isoform 1 of PIG-Y.
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Purification
- PIG-Y Antibody is affinity chromatography purified via peptide column.
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Immunogène
- PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y.
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Isotype
- IgG
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Indications d'application
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PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PIG-Y Antibody is supplied in PBS containing 0.02 % sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C,4 °C
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Stockage commentaire
- PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- PIGY (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))
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Autre désignation
- PIG-Y
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Sujet
- PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
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Poids moléculaire
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Predicted: 8 kDa
Observed: 16 kDa -
ID gène
- 84992
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NCBI Accession
- NP_116295
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UniProt
- Q3MUY2
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Pathways
- Inositol Metabolic Process
Antigène
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