NOTUM anticorps (N-Term)

N° du produit ABIN6991166

L’anticorps anti-NOTUM Polyclonal Lapin est utilisé pour la détection de NOTUM dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IHC (p) et IF.

Aperçu rapide pour NOTUM anticorps (N-Term) (ABIN6991166)

Antigène: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOTUM est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-NOTUM anticorps

Épitope: AA 90-140, N-Term

Purification: NOTUM Antibody is affinity chromatography purified via peptide column.

Immunogène: NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM.

Isotype: IgG

Information d'application

Indications d'application: NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOTUM Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: NOTUM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur NOTUM

Antigène: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Autre désignation: NOTUM

Sujet: NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

ID gène: 147111

NCBI Accession: NP_848588

UniProt: Q6P988