GOLPH3 anticorps (N-Term)

N° du produit ABIN6991206

Cet anticorps anti-GOLPH3 est un anticorps Lapin Polyclonal détectant GOLPH3 dans WB, ELISA et IF. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour GOLPH3 anticorps (N-Term) (ABIN6991206)

Antigène: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GOLPH3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-GOLPH3 anticorps

Épitope: N-Term

Purification: GOLPH3 Antibody is affinity chromatography purified via peptide column.

Immunogène: GOLPH3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3. The immunogen is located within the first 50 amino acids of GOLPH3.

Isotype: IgG

Information d'application

Indications d'application: GOLPH3 antibody can be used for detection of GOLPH3 by Western blot at 0.5 - 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: GOLPH3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: GOLPH3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur GOLPH3

Antigène: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Autre désignation: GOLPH3

Sujet: GOLPH3 Antibody: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

ID gène: 64083

UniProt: Q9H4A6