NUMB anticorps (C-Term)

N° du produit ABIN6991533

Cet anticorps anti-NUMB est un anticorps Lapin Polyclonal détectant NUMB dans WB, ELISA, IF et IHC (p). Adapté pour Humain, Souris et Rat.

Aperçu rapide pour NUMB anticorps (C-Term) (ABIN6991533)

Antigène: NUMB (Numb Homolog (NUMB))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NUMB est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NUMB anticorps

Épitope: C-Term

Specificité: At least four isoforms of NUMB are known to exist, this antibody will detect all of them.

Purification: NUMB Antibody is affinity chromatography purified via peptide column.

Immunogène: NUMB antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human NUMB. The immunogen is located within the last 50 amino acids of NUMB.

Isotype: IgG

Information d'application

Indications d'application: NUMB antibody can be used for detection of NUMB by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: NUMB Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: NUMB antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur NUMB

Antigène: NUMB (Numb Homolog (NUMB))

Autre désignation: NUMB

Sujet: NUMB Antibody: NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

ID gène: 8650

NCBI Accession: NP_1005743

UniProt: P49757

Pathways: Cell-Cell Junction Organization