Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
Specificité
ECRG4 antibody is predicted to not cross-react with other ECRG family members.
Purification
ECRG4 Antibody is affinity chromatography purified via peptide column.
Immunogène
ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4. The immunogen is located within the last 50 amino acids of ECRG4.
C2orf40
Reactivité: Humain, Souris, Rat
WB, IHC (p)
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ECRG4 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
ECRG4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
C2orf40
(Chromosome 2 Open Reading Frame 40 (C2orf40))
anticorps ECRG4, anticorps ecrg4, anticorps ecrg4-A, anticorps Ecrg4, anticorps chromosome 2 open reading frame, human C2orf40, anticorps chromosome 13 open reading frame, human C2orf40, anticorps chromosome 11 open reading frame, human C2orf40, anticorps chromosome 2 open reading frame 40, anticorps chromosome 2 open reading frame 40 L homeolog, anticorps RIKEN cDNA 1500015O10 gene, anticorps similar to RIKEN cDNA 1500015O10, anticorps C2H2orf40, anticorps C13H2orf40, anticorps C11H2orf40, anticorps C2orf40, anticorps c2orf40.L, anticorps 1500015O10Rik, anticorps RGD1305645
Sujet
ECRG4 Antibody: The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.